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biotinylated goat anti mouse ig  (SouthernBiotech)


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    Structured Review

    SouthernBiotech biotinylated goat anti mouse ig

    Biotinylated Goat Anti Mouse Ig, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+mouse+ig/Goat+Anti-Mouse+Ig%2C+Human+ads-BIOT/pmc11417334-22-0-6
    Average 94 stars, based on 17 article reviews
    biotinylated goat anti mouse ig - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "T-follicular regulatory cells expand to control germinal center plasma cell output but fail to curb autoreactivity"

    Article Title: T-follicular regulatory cells expand to control germinal center plasma cell output but fail to curb autoreactivity

    Journal: iScience

    doi: 10.1016/j.isci.2024.110887


    Figure Legend Snippet:

    Techniques Used: Recombinant, Amplification, Staining, Software

    Related Articles

    Incubation:

    Article Title: Antigen presentation by B cells enables epitope spreading across an MHC barrier.
    Article Snippet: .. Wells were washed thrice in TBS/Tw and incubated with biotinylated goat-anti-mouse Ig (1010-08, Southern Biotech), 1 μg/ ml TBS/Tw, at 37 °C for 1 hour. .. Wells were washed 3 times in TBS/Tw, and Eu3+-labeled streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μMEDTAwas subsequently added to thewells and incubated at RT for 1 h. Finally, the wells were washed thrice with TBS/Tw, and 200 μL enhancement buffer (AMPQ99800, Amplicon) was added.

    Article Title: Pneumococcal surface protein A inhibits complement deposition on the pneumococcal surface by competing with the binding of C-reactive protein to cell-surface phosphocholine
    Article Snippet: .. To qualitatively detect PspA, 1/4 serial dilutions of cell eluates were immunoblotted on nitrocellulose membranes, blocked with 1% BSA in PBS, incubated with PspA specific mAb, XiR278 ( 50 ), and developed with biotinylated goat anti-mouse Ig, alkaline phosphatase conjugated streptavidin (Southern Biotechnology Associates Inc., Birmingham, AL) and nitrobluetetrazolium substrate with 5-bromo-4-chloro-3-indoyl phosphate p -toluedene salt (Fisher Scientific, Norcross, GA). ..

    Article Title: B cell MHC haplotype affects follicular inclusion, germinal center participation and plasma cell differentiation in a mouse model of lupus
    Article Snippet: .. Wells were washed thrice in TBS/Tw and incubated with biotinylated goat-anti-mouse Ig (1010-08, Southern Biotech), 1 μg/ml TBS/Tw, at 37°C for 1 hour. .. Wells were washed 3 times in TBS/Tw, and Eu 3+ -labeled streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA was subsequently added to the wells and incubated at RT for 1 hour.

    Article Title: Single and Coexpression of CXCR4 and CXCR5 Identifies CD4 T Helper Cells in Distinct Lymph Node Niches during Influenza Virus Infection
    Article Snippet: Briefly, Maxisorb plates (Thermo Scientific) were coated with 1,000 HAU of the relevant influenza virus ( 13 ). .. Plates were blocked, and tissue culture supernatants were prediluted 2.5-fold, added to the plate, 2-fold serially diluted in PBS, and incubated for 3 h. Antibody binding was revealed by using biotinylated goat anti-mouse Ig (H+L; Southern Biotech), streptavidin-horseradish peroxidase (Vector Labs), and TMB substrate ( 13 ). ..

    Article Title: Antigen presentation by B cells enables epitope spreading across an MHC barrier
    Article Snippet: .. Wells were washed thrice in TBS/Tw and incubated with biotinylated goat-anti-mouse Ig (1010-08, Southern Biotech), 1 μg/ml TBS/Tw, at 37 °C for 1 hour. .. Wells were washed 3 times in TBS/Tw, and Eu 3+ -labeled streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA was subsequently added to the wells and incubated at RT for 1 h. Finally, the wells were washed thrice with TBS/Tw, and 200 μL enhancement buffer (AMPQ99800, Amplicon) was added.

    Article Title: The diversity of the proline-rich domain of pneumococcal surface protein A (PspA): potential relevance to a broad-spectrum vaccine.
    Article Snippet: .. The membranes were next washed three times with 1XPBS incubated with either biotinylated goat anti-mouse Ig or goat anti-human IgG (for mouse monoclonal and human serum respectively) and alkaline phosphatase-conjugated streptavidin (Southern Biotechnology Associates, Birmingham, AL) for 1 hour at room temperature, washed and developed with nitroblue tetrazolium substrate with 5-bromo-4-chloro-3-indoyl phosphate p-toluidine salt (Fisher Scientific, Norcross, GA) to qualitatively determine antibody binding to PRD and αHD portions of PspA. ..

    other:

    Article Title: T-follicular regulatory cells expand to control germinal center plasma cell output but fail to curb autoreactivity
    Article Snippet: Biotinylated goat- anti -mouse Ig , Southern Biotech , Cat#1010-08.

    Binding Assay:

    Article Title: Single and Coexpression of CXCR4 and CXCR5 Identifies CD4 T Helper Cells in Distinct Lymph Node Niches during Influenza Virus Infection
    Article Snippet: Briefly, Maxisorb plates (Thermo Scientific) were coated with 1,000 HAU of the relevant influenza virus ( 13 ). .. Plates were blocked, and tissue culture supernatants were prediluted 2.5-fold, added to the plate, 2-fold serially diluted in PBS, and incubated for 3 h. Antibody binding was revealed by using biotinylated goat anti-mouse Ig (H+L; Southern Biotech), streptavidin-horseradish peroxidase (Vector Labs), and TMB substrate ( 13 ). ..

    Article Title: The diversity of the proline-rich domain of pneumococcal surface protein A (PspA): potential relevance to a broad-spectrum vaccine.
    Article Snippet: .. The membranes were next washed three times with 1XPBS incubated with either biotinylated goat anti-mouse Ig or goat anti-human IgG (for mouse monoclonal and human serum respectively) and alkaline phosphatase-conjugated streptavidin (Southern Biotechnology Associates, Birmingham, AL) for 1 hour at room temperature, washed and developed with nitroblue tetrazolium substrate with 5-bromo-4-chloro-3-indoyl phosphate p-toluidine salt (Fisher Scientific, Norcross, GA) to qualitatively determine antibody binding to PRD and αHD portions of PspA. ..



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    Image Search Results


    Journal: iScience

    Article Title: T-follicular regulatory cells expand to control germinal center plasma cell output but fail to curb autoreactivity

    doi: 10.1016/j.isci.2024.110887

    Figure Lengend Snippet:

    Article Snippet: Biotinylated goat- anti -mouse Ig , Southern Biotech , Cat#1010-08.

    Techniques: Recombinant, Amplification, Staining, Software

    Journal: iScience

    Article Title: T-follicular regulatory cells expand to control germinal center plasma cell output but fail to curb autoreactivity

    doi: 10.1016/j.isci.2024.110887

    Figure Lengend Snippet:

    Article Snippet: The plate was incubated at 37°C for 1 h. Wells were washed three times in TBS/Tw and incubated with biotinylated goat- anti -mouse Ig (1010-08, Southern Biotech), 1 μg/mL TBS/Tw, at 37°C for 1 h. Wells were washed 3 times in TBS/Tw, and Eu 3+ -labeled streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA was subsequently added to the wells and incubated at RT for 1 h. Finally, the wells were washed three times with TBS/Tw, and 200 μL enhancement buffer (AMPQ99800, Amplicon) was added.

    Techniques: Recombinant, Amplification, Staining, Software